Quantitative Genotyping Analysis:
Pyrosequencing technology offers the unique advantage over other genotyping methods of providing quantitative measurements of
every allele.  This property makes it a good choice for SNP genotyping in DNA pools for association/linkage analysis studies and for
loss of heterozygosity studies.  Additional application areas include gene copy determination and analysis of mutations in mixed
populations in tumor and normal cells.
Applications:
  •        Genotyping of sample pools for association/linkage analysis
  •        Allele specific expression analysis
  •        Loss of heterozygosity (LOH)
  •        Gene copy number determination.
  •        Mutation detection in mixed populations
The services include:
Items
 Service Products
 Description
 Order
8024
 PCR amplification
 PCR from genomic DNA for Pyrosequencing allele
quantification analysis
 Inquiry/
Order
8009
 Allele quantification
 Pyrosequencing quantitative genotyping and allele
quantification analysis
 Inquiry/
Order
8006
 Assay Design - Standard
 In silico assay design for PCR and Pyrosequencing analysis.  
Customers will submit the DNA sequence containing the
target region of interest.  We deliver an assay design report
with recommendations for PCR and Pyrosequencing primers.  
Customer will run PCR and Pyrosequencing based on our
design.
 Inquiry/
Order
8007
 Assay Design - Gold
 Assay design for Pyrosequencing analysis.   
Customers submit the DNA sequence containing the target
region of interest.  We deliver an assay design report
including PCR and Pyrosequencing primers.
Customers will run PCR based on our design and send the
PCR product to us for Pyrosequencing analysis.
 Inquiry/
Order
8008
 Assay Design - Premium
 Assay design and assay optimization for Pyroseqencing
analysis.  
Customers submit the DNA sequence containing target region
of interest.  We optimize the PCR and Pyrosequencing assay
on 10 DNA samples or controls and  deliver an assay design
report with PCR and Pyrosequencing primers and assay
conditions along with performance data.
 Inquiry/
Order
Tel. - 1 508.852.4200 · Fax - 1 508.852.4202 · email - info@epigendx.com
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Solutions for Genomic
and Epigenomic Research