EpigenDx - Quantitative genotyping - Allele specific expression

Solutions for Genomic
and Epigenomic Research

Allele-Specific Expression Analysis

It has been known that allele specific expression one of the two parental alleles is associated with epigenetic phenomena of
X-chromosome inactivation and genomic imprinting. Allele specific differences in gene expression are also found in non-imprinted
autosomal genes. A well established method of Quantifying allele-specific expression uses a transcribed SNP to distinguish
betweens alleles in cells that are heterozygous. Pyrosequencing technology is an ideal method for identifying heterozygous SNPs
and quantifying alleles in transcripts in different cells and tissues.

Applications:

Systematic screening for imprinted genest.

Quantification of allele-specific transcripts in different cells and tissues.

Our services

We offer high throughput SNP genotyping analysis

We offer accurate allele quantification analysis

We deliver quantitative genotyping results in % of each allele

Analysis Results

Example

For more information, please contact us.

References

Expression profiling of uniparental mouse embryos is in inefficient in identifying novel imprinted genes Genomics
87 (2006) 509-519

Sequence-based bioinformatic prediction and QUASEP identify genomic imprinting of the KCNK9 potassium channel
gene in mouse and human Hum Mol Genet. 2007 Aug 17

Detection of allelic imbalance in gene expression using pyrosequencing Methods Mol Biol. 2007;373:157-76

Quantification of allele-specific G-protein beta3 subunit mRNA transcripts in different human cells and tissues by
Pyrosequencing Eur J Hum Genet. 2005 Mar;13(3):361-9