CpG Methylation Analysis
DNA methylation refers to the covalent addition of a methyl group to the 5-carbon of cytosine in a CpG dinucleotide.  It is an
epigenetic event that affects cell function by altering gene expression.  Methods for DNA methylation analysis can be divided into
two categories: global and gene-specific methylation analysis.
Bisulfite Pyrosequencing is a sequencing-based analysis of DNA methylation that quantitatively measures multiple, consecutive
CpG sites individually with high accuracy and reproducibility.  We offer DNA methylation services for both global and gene-specific
methylation analysis using Pyrosequencing technology.  For more information or to submit the DNA sequence information with
target region of interest highlighted, please send an email to  info@epigendx.com.
Global DNA Methylation Analysis:
        Rat ID Element Methylation Analysis:  
       Rat Line-1 Methylation Analysis  Long interspersed elements make up about 15% of rat genome and contain much
of the CpG methylation found in normal postnatal somatic tissues...   
The services include:
Items
 Service Products
 Description
 Order
8001
 DNA Methylation Analysis - Standard
 Quantitative Methylation Analysis on submitted PCR products
  • Perform  Pyrosequencing methylation analysis
 Inquiry/
Order
8002
 DNA Methylation Analysis - Gold
 Quantitative Methylation Analysis on submitted bisulfite modified DNA
  • Perform PCR reaction
  • Perform Pyrosequencing methylation analysis
 Inquiry/
Order
8003
 DNA Methylation Analysis - Premium
 Quantitative Methylation Analysis on submitted genomic DNA
  • Perform bisulfite modification
  • Perform PCR reactions
  • Perform Pyrosequencing analysis
 Inquiry/
Order
8005
 Bisulfite modification
 Bisulfite modification on submitted genomic DNA
  • Process extracted genomic DNA from different sample
    sources, such as paraffin embedded tissues, frozen tissues,
    serum, and more
 Inquiry/
Order
8006
 Assay Design - Standard
 Pyrosequencing primer design for DNA methylation, SNP genotyping,
and allele specific expression analysis:  
  • Customer provides target sequences
  • Customer designs PCR primers
  • EpigenDx determines validity of the PCR primers, designs
    Pyrosequencing primers, and determines the biotin labeled
    PCR primers.
 Inquiry/
Order
8008
 Assay Design - Premium
 Design and optimize PCR and Pyrosequencing primers for DNA
methylation analysis, SNP genotyping, and allele specific expression
analysis:  
  • Design PCR and Pyrosequencing primers
  • Develop and optimize PCR condition
  • Develop and optimize Pyrosequencing condition
  • Perform assay validation
 Inquiry/
Order
Tel. - 1 508.852.4200 · Fax - 1 508.852.4202 · email - info@epigendx.com
Pyrosequencing and Pyrogram are registered trademarks of Biotage.
© Copyright 2006 EpigenDx.
Learn More about Pyrosequencing >>
Solutions for Genomic
and Epigenomic Research
Gene Specific DNA Methylation Analysis:
        P16 Methylation Assay:  We use PyroMark RUO kit or home brew methylation for analyzing epigenetic changes in
the p16 (CDKN2A) protomoter.  
       MGMT Methylation Assay:  We have assays to analyze MGMT promoter and MGMT exon 1.  To analyze MGMT
promoter regions, we use Biotage's PyroMark MGMT RUO kit and in-house developed assays to cover 28 CpG sites.  
Assay for MGMT exon 1 covers 5 CpG sites.  
  •        Human Global Methylation Analysis Assays:
  •        Rat Global Methylation Analysis Assays:
  •        Mouse Global Methylation Analysis Assays:
       Human Line-1 Methylation Analysis:  Long interspersed elements make up about 15% of human genome and
contain much of the CpG methylation found in normal postnatal somatic tissues...   Click Here for more information about
this assay.  
       Human Alu Repetitive Elements:  Alu repeat is the most plentiful short interspersed nucleotide element in human
DNA.  It comprises 10% of the human genome.
        Mouse B1 Repetitive Element:  There are 30,000 copies of the B1 element are randomly dispersed in the total
mouse  genome ...   Click Here for more information about this assay.  
       Mouse Line-1 Methylation Analysis:  Long interspersed elements make up about 15% of mouse genome and
contain much of the CpG methylation found in normal postnatal somatic tissues...