CpG Methylation Analysis
DNA methylation refers to the covalent addition of a methyl group to the 5-carbon of cytosine in a CpG dinucleotide.  It is an
epigenetic event that affects cell function by altering gene expression.  Methods for DNA methylation analysis can be divided into
two categories: global and gene-specific methylation analysis.
Bisulfite Pyrosequencing is a sequencing-based analysis of DNA methylation that quantitatively measures multiple, consecutive
CpG sites individually with high accuracy and reproducibility.  We offer DNA methylation services for both global and gene-specific
methylation analysis using Pyrosequencing technology.  For more information or to submit the DNA sequence information with
target region of interest highlighted, please send an email to  info@epigendx.com.
Global DNA Methylation Analysis:
  •        Mouse B1 Repetitive Element:  There are 30,000 copies of the B1 element are randomly dispersed in the total
    mouse  genome ...   Click Here for more information about this assay.  
  •        Human Line-1 Biotage's RUO Kit:  Long interspersed elements make up about 15% of human genome and contain
    much of the CpG methylation found in normal postnatal somatic tissues...   Click Here for more information about this
    assay.  
  •        Human Alu Repetitive Elements:  Alu repeat is the most plentiful short interspersed nucleotide element in human
    DNA.  It comprises 10% of the human genome.
The services include:
Items
 Service Products
 Description
 Order
8001
 DNA Methylation Analysis - Standard
 Quantitative Methylation Analysis on submitted PCR products
  • Perform  Pyrosequencing methylation analysis
 Inquiry/
Order
8002
 DNA Methylation Analysis - Gold
 Quantitative Methylation Analysis on submitted bisulfite modified DNA
  • Perform PCR reaction
  • Perform Pyrosequencing methylation analysis
 Inquiry/
Order
8003
 DNA Methylation Analysis - Premium
 Quantitative Methylation Analysis on submitted genomic DNA
  • Perform bisulfite modification
  • Perform PCR reactions
  • Perform Pyrosequencing analysis
 Inquiry/
Order
8005
 Bisulfite modification
 Bisulfite modification on submitted genomic DNA
  • Accept extracted genomic DNA from different sample sources,
    such as paraffin embedded tissues, frozen tissues, serum,
    and more
 Inquiry/
Order
8006
 Assay Design - Standard
 Design PCR and Pyrosequencing primers for DNA methylation, SNP
genotyping, and allele specific expression analysis:  
  • Provide in silico designed primer sequencing information
 Inquiry/
Order
8007
 Assay Design - Gold
 Design PCR and Pyrosequencing primers for DNA methylation
analysis, SNP genotyping, and allele specific expression analysis:
  • Provide in silico designed primer information
  • Perform Pyrosequencing analysis on submitted PCR product
 Inquiry/
Order
8008
 Assay Design - Premium
 Design and optimize PCR and Pyrosequencing primers for DNA
methylation analysis, SNP genotyping, and allele specific expression
analysis:  
  • Design PCR and Pyrosequencing primers
  • Develop and optimize PCR condition
  • Develop and optimize Pyrosequencing condition
  • Perform assay validation
 Inquiry/
Order
Tel. - 1 508.852.4200 · Fax - 1 508.852.4202 · email - info@epigendx.com
Pyrosequencing and Pyrogram are registered trademarks of Biotage.
© Copyright 2006 EpigenDx.
Learn More about Pyrosequencing >>
Solutions for Genomic
and Epigenomic Research
Gene Specific DNA Methylation Analysis:
  •         P16 Methylation Assay:  We use Biotage's PyroMark RUO kit for P16 methylation analysis.  
  •        MGMT Methylation Assay:  We have assays to analysis MGMT promoter and MGMT exon 1.  To analyze MGMT
    promoter regions, we use Biotage's PyroMark MGMT RUO kit and in-house developed assays to cover 28 CpG sites.  
    Assay for MGMT exon 1 covers 5 CpG sites.