Allele-Specific Expression Analysis
- It has been known that allele specific expression one of the two parental alleles is associated with epigenetic phenomena of X-chromosome inactivation and genomic imprinting. Allele specific differences in gene expression are also found in non-imprinted autosomal genes. A well established method of Quantifying allele-specific expression uses a transcribed SNP to distinguish betweens alleles in cells that are heterozygous. Pyrosequencing technology is an ideal method for identifying heterozygous SNPs and quantifying alleles in transcripts in different cells and tissues.
Applications:
- Systematic screening for imprinted genes.
- Quantification of allele-specific transcripts in different cells and tissues.
- We offer high throughput SNP genotyping analysis
- We offer accurate allele quantification analysis
- We deliver quantitative genotyping results in % of each allele
| Example |  |
- Expression profiling of uniparental mouse embryos is in inefficient in identifying novel imprinted genes Genomics 87 (2006) 509-519
- Sequence-based bioinformatic prediction and QUASEP identify genomic imprinting of the KCNK9 potassium channel gene in mouse and human Hum Mol Genet. 2007 Aug 17
- Detection of allelic imbalance in gene expression using pyrosequencing Methods Mol Biol. 2007;373:157-76
- Quantification of allele-specific G-protein beta3 subunit mRNA transcripts in different human cells and tissues by Pyrosequencing Eur J Hum Genet. 2005 Mar;13(3):361-9
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