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DNA Methylation Analysis - Validated PCR Primer Sets:

  • Carefully designed using unique methylation analysis assay design algorithm.
  • Validated using quantitative Pyrosequencing methylation analysis technology.
  • Tested to ensure the methylated and unmethylated DNA are amplified at same efficiency.
  • Ideal for quantitative Pyrosequencing DNA methylation analysis.
  • Good for bisulfite subcloning sequencing.
  • Many assays work for combined bisulfite restriction analysis (COBRA).
  • Available for gene specific methylation analysis of human, mouse, and rat DNA samples.
  • Perform up to 300 reactions (30 µl of 100 µM primer solution, forward and reverse PCR primers).
  • Pyrosequencing primers available for the Pyrosequencer user.
  • Assay conditions and Pyrosequencing QC results included.

Control DNA for Bisulfite Methylation Analysis:

  • Highly methylated DNA is in vitro enzymatically methylated genomic DNA.
  • Low methylated DNA is chemically and enzymatically treated genomic DNA.
  • Control DNA is intended for serving as positive or negative control in DNA methylation analysis.
  • Tested on numerous gene-specific and global methylation assays.
  • Available for human, mouse, and rat DNA methylation analysis assays.
  • Available as pre-mixed calibration standards.

Products include:

Items Description Price Order
80-8061-HGHM5 Human highly methylated genomic DNA (5 µg at 100 ng/ µL) $173.61 Inquiry / Order
80-8062-HGUM5 Human low methylated genomic DNA (5 µg at 100 ng/ µL) $173.61 Inquiry / Order
80-8063-MGHM5 Mouse highly methylated genomic DNA (5 µg at 100 ng/ µL) $173.61 Inquiry / Order
80-8064-MGUM5 Mouse low methylated genomic DNA (5 µg at 100 ng/ µL) $173.61 Inquiry / Order
80-8065-RGHM5 Rat highly methylated genomic DNA (5 µg at 100 ng/ µL) $173.61 Inquiry / Order
80-8066-RGUM5 Rat low methylated genomic DNA (5 µg at 100 ng/ µL) $173.61 Inquiry / Order
80-8060R-PreMix Rat Premixed Calibration Standard (1 µg per vial at different ratios) $281.14 Inquiry / Order
80-8060M-PreMix Mouse Premixed Calibration Standard (1 µg per vial at different ratios) $281.14 Inquiry / Order
80-8060H-PreMix Human Premixed Calibration Standard (1 µg per vial at different ratios) $281.14 Inquiry / Order
80-8080-ADS Validated Methylated Analysis Primer Set (Including assay conditions and a primer set good for 300 reactions) $231.52 Inquiry / Order