Pyosequencing is an Effective Method for the Quantitative Analysis of Global and Gene-specific DNA Methylation Using Low Quantities of DNA

Because DNA isolated from clinical blood serum samples are treated in different ways and the quantity is often limiting, it is important to show that consistent methylation results can be obtained with small quantities of DNA. In this study we quantitatively analyzed both global and gene specific methylation of DNA isolated from different sera samples. Additionally, we analyzed DNA from cells that were sorted in 2-fold decreasing amounts to determine the limit of sensitivity of a variety of assays. Among the genes we analyzed were the Alu and Line-1 elements for global methylation and gene specific MGMT, p16, and RASSF1 assays. We demonstrated that Pyrosequencing® methylation analysis achieves a high degree of consistency and sensitivity when used on DNA isolated from blood serum and different assays differ in their lower limit of DNA that can be analyzed.

To request a copy, Please contact: info@epigendx.com

• The Use of Pyrosequencing as a Valuable Tool for Detecting Epigenetic and Genetic Alterations in Ovarian Cancer Cell Lines (TT24)
• Pyrosequencing Methylation Profile of Seven Thyroid Cancer Cell Lines (Poster number ST07)
• Pyrosequencing Methylation Profile of Five Breast Cancer Cell Lines (Poster number ST09)